The pursuit started around 10 p.m. Friday, Minneapolis police said in a statement.
An officer spotted, and attempted to stop the driver speeding on Interstate 35.
The driver exited the highway before crashing into another vehicle with four women and a girl inside, police said.
All five were pronounced dead at the scene.
Police said the fleeing motorist then ran from the crash scene.
Officers searched the area before taking a suspect into custody. He was taken to a hospital to be evaluated.
Police haven’t released his name or the names of the victims. A homicide team is investigating.
Adapted from reporting by the Associated Press